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OUR SUPPLIERS
KOMABIOTECH
301,
Gayang Technotown, #1487
Gayang 3 dong,
Gangseo-gu
Seoul 157-793, KOREA
Spherotech, Inc.
27845 Irma Lee Circle, Unit 101
Lake Forest, IL 60045
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Exalpha
Biologicals,
Inc.
2 Shaker Road,
Unit B101
Shirley, MA
01464
SCETI K.K
BIOSCIENCE Export DF Kasumigaseki Place,3-6-7 Kasumigaseki Chiyoda-ku, Tokyo 100-0013 JAPAN
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EY
Laboratories, Inc. Headquarters
107 N.
Amphlett Blvd
San Mateo, CA. 94401 USA
EXBIO Praha, a.s.
Nad
Safinou II 366
252 42 Vestec
Czech Republic
Sacace Biotechnologies S.r.l.
Via Scalabrini,
44
22100 Como Italy
GENTAUR BVBA
VAT BE0473327336
Av. de l Armee 68 B4
1040 Brussels
BELGIUM
Tel + 32 16 58 90
45
Fax + 32 16 50 90 45
GENTAUR France SARL
SIRET 48423788800017
Rue Lagrange, 9
75005 Paris,
France
Tel 01 43 25 01 50
Fax 01 43 25 01 60
GENTAUR Germany
Marienbongard 20
52074 Aachen,
Germany
Tel 0241 56 00
99 68
Fax 0241 56 00 47 88
 GENTAUR
Pol Sp. Z.o.o. Ulica
Ogarna 15/19B m2
80-826 GDANSK
Tel 00 48 58 760 77
08
Fax: 00 32 16 50 90
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GENTAUR Italy
23015 Milano, Italy
Tel 02 36 00 65
93
Fax 02 36 00 65
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Česká republika
Praha
+420246019719
Danmark
+4569918806
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+358942419041
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+35316526556
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+35220880274
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+3619980547
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+31208080893
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+4721031366
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+43720880899
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+46852503438
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+41435006251
Northern America
Canada Montreal
+15149077481
US New York
+17185132983
Other Countries
0032 (0)16 41 44 07 |
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ACT 1™
Assay for CytoToxicity
A non-radioactive cytotoxicity assay for flow cytometry
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| Key Benefits: |
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No radioactive materials required
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Applications - Works with a flow cytometer or
fluorescence microscope
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Detects cytolytic activity at a cellular level
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Works with multiple types of mammalian cell lines
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| Technology: |
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The most commonly used method to measure CMC/ADCC is a
radioactive chrominum-51 (51Cr)
release assays (2). There are several disadvantages with
this assay: it is expensive, difficult to load certain cell
types, expensive to dispose of due to strict environmental
regulations, and has high background from spontaneous
release of 51Cr. With
the use of flow cytometry, it is now possible to eliminate
the need for radioactive material and increase the ability
to quantify cytolytic activity on a single cell bases.
Various groups have demonstrated that measuring CMC/ADCC
activity by flow cytometry has a strong (95%) correlation
with the traditional 51Cr
release assay (3,4,5,6).
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| Assay Principle : |
A cell tracking dye CFSE ( 7,8,9) is utilized to label the
target cell population. After the assay has run its
experimental protocol, 7AAD (live/dead) (10,11) is added to
measure cell death. 7AAD only enters membrane-compromised
cells and binds to DNA.
Flow cytometry is utilized to gate on the target cells
and measure 7AAD negative vs 7AAD postitve cells. %
cytotoxicity is calculated by the following equation (see
experimental example below):
7AAD positive (upper right quadrant)=R1/
7AAD Postitve (upper right quadrant)= R1 +
7AAD negative (lower right quadrant)=R2
x100 |
| Kit contents: |
- 4 vials of CFSE membrane stain, part no 4002.
- 3 vials of 7AAD Live/Dead stain, part no 4003.
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| The following kit
is available: |
Product
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Catalog No.
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Size
(No. of Tests) |
Price |
ACT 1
Assay for CytoToxicity
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ACT100-2 |
100 |
€275 |
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